HOMER3 Human

Data sheet

Formulation

The HOMER3 solution (1mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 150mM NaCl, 1mM DTT and 30% glycerol.

Purity

Greater than 90% as determined by SDS-PAGE.

Inactivation

Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 1.13 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of

Description

HOMER3 Human Recombinant produced in E. coli is a single polypeptide chain containing 386 amino acids (1-361) and having a molecular mass of 42.5kDa.HOMER3 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniq

Protein Background

HOMER3 belongs to the HOMER family of postsynaptic density scaffolding proteins which have an analogous domain structure consisting of an N-terminal Enabled/vasodilator-stimulated phosphoprotein homology 1 domain that facilitates protein-protein relations

Expression host

E.coli.

Synonyms

Homer homolog 3 (Drosophila)1 2 , HOMER-31 2 , homer protein homolog 32 , Homer neuronal immediate early gene 32, Homer-33, VESL32.

Reagent Appearance

Sterile Filtered colorless solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSHMMSTAR EQPIFSTRAH VFQIDPATKR NWIPAGKHAL TVSYFYDATR NVYRIISIGG AKAIINSTVT PNMTFTKTSQ KFGQWADSRA NTVYGLGFAS EQHLTQFAEK FQEVKEAARL AREKSQDGGE LTSPALGLAS HQVPPSPLVS ANGPGEEKLF RSQSADAPGP TERERLKKML SEGSVGEVQW EAEFFALQDS NNKLAGALRE AN