| Formulation |
HMGN3 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH7.0) and 10% glycerol. |
| Purity |
Greater than 95% as determined by SDS-PAGE. |
| Description |
HMGN3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 100 amino acids (1-77 a.a) and having a molecular mass of 10.8 kDa. HMGN3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
| Protein Background |
High Mobility Group Nucleosomal Binding Domain 3 (HMGN3), binds thyroid hormone receptor beta, however it occurs only in the presence of thyroid hormone. Thyroid hormone receptors are hormone-dependent transcription factors which regulate expression of a variety of particular target genes. HMGN3 is considered to reduce the compactness of the chromatin fiber in nucleosomes, in that way enhancing transcription from chromatin templates. |
| Expression host |
Escherichia Coli. |
| Synonyms |
High Mobility Group Nucleosomal Binding Domain 3, TRIP7, TR-Interacting Protein 7, High Mobility Group Nucleosome-Binding Domain-Containing Protein 3, Thyroid Hormone Receptor Interacting Protein 7, Thyroid Receptor-Interacting Protein 7, Thyroid Hormone Receptor Interactor 7, PNAS-24, PNAS-25, TRIP-7, High mobility group nucleosome-binding domain-containing protein 3. |
| Reagent Appearance |
Sterile filtered colorless solution. |
| Stability |
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Amino acid sequence |
MGSSHHHHHH SSGLVPRGSH MGSMPKRKSP ENTEGKDGSK VTKQEPTRRS ARLSAKPAPP KPEPKPRKTS AKKEPGAKIS RGAKGKKEEK QEAGKEGTEN. |
