GNB3 Human

Data sheet

Formulation

GNB3 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 10% glycerol and 0.4M Urea.

Purity

Greater than 80.0% as determined by SDS-PAGE.

Description

GNB3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 363 amino acids (1-340 a.a) and having a molecular mass of 39.6kDa.GNB3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chroma

Protein Background

GNB3 belongs to the WD repeat G protein beta family. GNB3 is an important regulator of alpha subunit, as well as of certain signal transduction receptor and effector. A single-nucleotide polymorphism (C825T) in GNB3 is linked with essential hypertension a

Expression host

Escherichia Coli.

Synonyms

Transducin Beta Chain 3, G Protein, Beta-3 Subunit, GTP-Binding Regulatory Protein Beta-3 Chain, Guanine Nucleotide-Binding Protein G(I)/G(S)/G(T) Beta Subunit 3, Guanine Nucleotide-Binding Protein G(I)/G(S)/G(T) Subunit Beta-3, Hypertension Associated Pr

Reagent Appearance

Sterile Filtered clear solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSMGEMEQL RQEAEQLKKQ IADARKACAD VTLAELVSGL EVVGRVQMRT RRTLRGHLAK IYAMHWATDS KLLVSASQDG KLIVWDSYTT NKVHAIPLRS SWVMTCAYAP SGNFVACGGL DNMCSIYNLK SREGNVKVSR ELSAHTGYLS CCRFLDDNNI VTSSGDTTCA LWDIETGQQK TVFVGHTGDC MSLAVSPDFN LFISGACDAS AK