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Gly m 5.0101
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Formulation | Gly m 5.0101 is supplied in 20mM HEPES buffer pH-7.6, 250mM NaCl and 20% glycerol. |
Purity | Greater than 80.0% as determined by SDS-PAGE. |
Description | Recombinant Alpha Subunit of Beta Conglycinin produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 70,586 Dalton. Gly m 5.0101 is expressed with a 10xHis tag at N-terminus and purified by proprietary chromatographic techniques. |
Protein Background | Alpha Subunit of Beta Conglycinin (Gly m 5.0101) causes an allergic reaction in humans. |
Expression host | Sf9 insect cells. |
Synonyms | Alpha subunit of beta conglycinin, Gly m 5. |
Reagent Appearance | Sterile Filtered clear solution. |
Stability | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles. |
Immunological functions | 1. Binds IgE type human antibodies. 2. Immunodot test with positive/negative sera panels. |
Molar extinction coefficient | 35090; A280(1mg/ml)=0.497 |