| Formulation |
The glpE solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0) and 10% glycerol. |
| Purity |
Greater than 95.0% as determined by SDS-PAGE. |
| Description |
glpE Recombinant produced in E. coli is a single polypeptide chain containing 131 amino acids (1-108) and having a molecular mass of 14.5kDa. glpE is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
| Protein Background |
Thiosulfate sulfurtransferase (glpE) is a mitochondrial matrix enzyme which is encoded by the nucleus. Escherichia coli glpE is a prototype for the single-domain rhodanese superfamily. glpE catalyzes the sulfur-transfer reaction in which a sulfur atom is transferred from thiosulfate to cyanide by a double-displacement mechanism. |
| Expression host |
Escherichia Coli. |
| Synonyms |
ECK3411, JW3388, b3425, Thiosulfate sulfurtransferase GlpE. |
| Reagent Appearance |
Sterile Filtered colorless solution. |
| Stability |
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Amino acid sequence |
MGSSHHHHHH SSGLVPRGSH MGSMDQFECI NVADAHQKLQ EKEAVLVDIR DPQSFAMGHA VQAFHLTNDT LGAFMRDNDF DTPVMVMCYH GNSSKGAAQY LLQQGYDVVY SIDGGFEAWQ RQFPAEVAYG A. |
| Contaminents |
It is recommended to reconstitute the lyophilized Haptoglobin in sterile water not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
