FNDC5 Human

Data sheet

Formulation

FNDC5 filtered (0.4 um) and lyophilized from 0.5mg/ml in phosphate buffered saline.

Solubility

It is recommended to add 200ul deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. FNDC5 is not sterile! Please filter the product by an appropriate sterile filter before using

Purity

Greater than 95.0% as determined by SDS-PAGE.

Description

FNDC5 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (a.a 32-143) containing 122 amino acids including a 10 a.a N-terminal His tag. The total molecular mass is 13.8kDa (calculated).

Protein Background

Fibronectin Type III Domain Containing 5 (Irisin) is a newly discovered hormone, secreted into circulation by muscle, which acts on white adipocytes and promotes WAT browning. r-Irisin can reduce body weight and cause induction of brown adipocyte in vivo,

Expression host

Escherichia Coli.

Synonyms

Fibronectin type III domain-containing protein 5, Fibronectin type III repeat-containing protein, Fibronectin Type III Repeat-Containing Protein 2, Irisin, FNDC5, FRCP2.

Reagent Appearance

Filtered White lyophilized (freeze-dried) powder.

Stability

Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

Amino acid sequence

MKHHHHHHASDSPSAPVNVT VRHLKANSAV VSWDVLEDEV VIGFAISQQK KDVRMLRFIQ EVNTTTRSCA LWDLEEDTEY IVHVQAISIQ GQSPASEPVL FKTPREAEKM ASKNKDEVTM KE.