FAM84B Human

Data sheet

Formulation

The FAM84B solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 10% glycerol, 0.15M NaCl, 1mM DTT and 0.1mM PMSF.

Purity

Greater than 85% as determined by SDS-PAGE.

Description

FAM84B Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 333 amino acids (1-310) and having a molecular mass of 36.9kDa.FAM84B is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chroma

Protein Background

Family with Sequence Similarity 84, Member B (FAM84B) coprecipitated with a downstream effector of RAS, CRAF. Binding of FAM83B with CRAF interrupted CRAF/14-3-3 interactions and increased CRAF membrane localization, causing elevated MAPK and mammalian ta

Expression host

Escherichia Coli.

Synonyms

FAM84B, Family with Sequence Similarity 84 Member B, BCMP101, Breast Cancer Membrane-Associated Protein 101, Neurological/Sensory 2, Breast Cancer Membrane Protein 101, NSE2, Protein FAM84B, Protein NSE2.

Reagent Appearance

Sterile filtered colorless solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSMGNQVEK LTHLSYKEVP TADPTGVDRD DGPRIGVSYI FSNDDEDVEP QPPPQGPDGG GLPDGGDGPP PPQPQPYDPR LHEVECSVFY RDECIYQKSF APGSAALSTY TPENLLNKCK PGDLVEFVSQ AQYPHWAVYV GNFQVVHLHR LEVINSFLTD ASQGRRGRVV NDLYRYKPLS SSAVVRNALA HVGAKERELS WRNSESFAAW CR