| Formulation |
EXOSC3 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 7.5), 0.15M NaCl, 30% glycerol and 1mM DTT. |
| Purity |
Greater than 85.0% as determined by SDS-PAGE. |
| Description |
EXOSC3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 298 amino acids (1-275 a.a) and having a molecular mass of 32.0kDa. EXOSC3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
| Protein Background |
Exosome Component 3 (EXOSC3) is a non-catalytic component of the human exosome, a complex with 3'-5' exoribonuclease activity. EXOSC3 takes part in various RNA processing and degradation activities including degradation of histone mRNA. Linked pseudogenes of EXOSC3 are located on chromosome 19 and 21. |
| Expression host |
Escherichia Coli. |
| Synonyms |
bA3J10.7, CGI-102, hRrp-40, p10, PCH1B, RP11-3J10.8, RRP40, Rrp40p, Exosome complex component RRP40, Exosome component 3, Ribosomal RNA-processing protein 40, EXOSC3. |
| Reagent Appearance |
Sterile Filtered colorless solution. |
| Stability |
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Amino acid sequence |
MGSSHHHHHH SSGLVPRGSH MGSMAEPASV AAESLAGSRA RAARTVLGQV VLPGEELLLP EQEDAEGPGG AVERPLSLNA RACSRVRVVC GPGLRRCGDR LLVTKCGRLR HKEPGSGSGG GVYWVDSQQK RYVPVKGDHV IGIVTAKSGD IFKVDVGGSE PASLSYLSFE GATKRNRPNV QVGDLIYGQF VVANKDMEPE MVCIDSCGRA NGMGVIGQDG LLFKVTLGLI RKLLAPDCEI IQEVGKLYPL EIVFGMNGRI WVKAKTIQQT LILANILEAC EHMTSDQRKQ IFSRLAES. |
