| Formulation |
The EMC2 solution contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 2mM DTT and 40% glycerol. |
| Purity |
Greater than 90% as determined by SDS-PAGE. |
| Description |
EMC2 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 320 amino acids (1-297) and having a molecular mass of 37.2kDa.EMC2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
| Protein Background |
EMC2 is an element of the ER membrane protein complex. EMC2 is a member of the EMC2 family and holds 3 TPR repeats. |
| Expression host |
Escherichia Coli. |
| Synonyms |
ER Membrane Protein Complex Subunit 2, KIAA0103, Tetratricopeptide Repeat Domain 35, Tetratricopeptide Repeat Protein 35, TPR Repeat Protein 35, TTC35. |
| Reagent Appearance |
Sterile filtered colorless solution. |
| Stability |
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Amino acid sequence |
MGSSHHHHHH SSGLVPRGSH MGSMAKVSEL YDVTWEEMRD KMRKWREENS RNSEQIVEVG EELINEYASK LGDDIWIIYE QVMIAALDYG RDDLALFCLQ ELRRQFPGSH RVKRLTGMRF EAMERYDDAI QLYDRILQED PTNTAARKRK IAIRKAQGKN VEAIRELNEY LEQFVGDQEA WHELAELYIN EHDYAKAAFC LEELMMTNPH NHLYCQQYAE VKYTQGGLEN LELSRKYFAQ ALKLNNRNMR ALFGLYMSAS HIASNPKASA KTKKDNMKYA SWAASQINRA YQFAGRSKKE TKYSLKAVED MLETLQITQS |
