Dengue Envelope-1, Insect

Data sheet

Formulation

Dengue Envelope-1 protein solution in 1xD-PBS, pH7.4, 0.1% Thimerosal, 5mM EDTA, 1ug/ml of Leupeptin, Aprotinin and Pepstatin A.

Purity

Protein is >95% pure as determined by 12.5% SDS-PAGE.

Description

Recombinant Dengue Virus Subtype 1 produced in Insect Cells is a polypeptide chain containing amino acids 281-675 and having a molecular weight of approximately 50kDa. Dengue Envelope-1 is purified by proprietary chromatographic technique.

Protein Background

Caused by one of four closely related virus serotypes of the genus Flavivirus, family Flaviviridae, each serotype is sufficiently different that there is no cross-protection and epidemics caused by multiple serotypes (hyperendemicity) can occur. In cell c

Expression host

Insect cells.

Reagent Appearance

Sterile filtered colorless solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Amino acid sequence

mrcvgignrd fveglsgatw vdvvlehgsc vttmakdkpt ldiellktev tnpavlrklc ieakisnttt dsrcptqgea tlveeqdanf vcrrtfvdrg wgngcglfgk gslltcakfk cvtklegkiv qyenlkysvi vtvhtgdqhq vgnettehgt iatitpqapt seiqltdyga ltldcsprtg ldfnemvllt mkekswlvhk qwfldlplpw tsgastsqet wn