DBR1 Human

Data sheet

Formulation

The DBR1 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 0.15M NaCl, 1mM DTT and 20% glycerol.

Purity

Greater than 90.0% as determined by SDS-PAGE.

Description

DBR1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 340 amino acids (228-544) and having a molecular mass of 38kDa (molecular size on SDS-PAGE will appear higher).DBR1 is fused to a 23 amino acid His-tag at

Protein Background

DBR1 is an RNA lariat debranching enzyme which hydrolyzes 2'-5' prime branched phosphodiester bonds. The encoded protein specifically targets the bonds at the branch point of eliminated lariat intron RNA, transforming them to linear molecules which are th

Expression host

Escherichia Coli.

Synonyms

Debranching RNA Lariats 1, Lariat Debranching Enzyme, Debranching Enzyme Homolog 1, RNA Lariat Debranching Enzyme, EC 3.1.-.-.

Reagent Appearance

Sterile Filtered clear solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSHVKFAAL MQHQAKDKGQ TARATKFLAL DKCLPHRDFL QILEIEHDPS APDYLEYDIE WLTILRATDD LINVTGRLWN MPENNGLHAR WDYSATEEGM KEVLEKLNHD LKVPCNFSVT AACYDPSKPQ TQMQLIHRIN PQTTEFCAQL GIIDINVRLQ KSKEEHHVCG EYEEQDDVES NDSGEDQSEY NTDTSALSSI NPDEIMLDEE ED