| Formulation |
CYP2E1 protein solution (0.5mg/ml) containing 20mM Tris-HCl (pH8.0) and 10% glycerol. |
| Purity |
Greater than 85.0% as determined by SDS-PAGE. |
| Description |
CYP2E1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 488 amino acids (29-493 a.a) and having a molecular mass of 56.2kDa. CYP2E1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
| Protein Background |
CYP2E1, also known as Cytochrome P450 2E1 belongs to the cytochrome P450 mixed-function oxidase system which is involved in the metabolism of xenobiotics in the body. While CYP2E1 is implicated in the oxidative metabolism of small range substrates, commonly small polar molecules, there are many significant drug interactions which are mediated by CYP2E1. |
| Expression host |
Escherichia Coli. |
| Synonyms |
Cytochrome P450, Family 2, Subfamily E, Polypeptide 1, CYP2E, Cytochrome P450, Subfamily IIE (Ethanol-Inducible), Polypeptide 1, 4-Nitrophenol 2-Hydroxylase, Cytochrome P450-J, P450C2E, CYPIIE1, Flavoprotein-Linked Monooxygenase, Microsomal Monooxygenase, Xenobiotic Monooxygenase, Cytochrome P450 2E1, EC 1.14.13.n7, EC 1.14.14.1, EC 1.14.13.-, P450-J, CPE1, Cytochrome P450 2E1. |
| Reagent Appearance |
Sterile filtered colorless solution. |
| Stability |
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Amino acid sequence |
MGSSHHHHHH SSGLVPRGSH MGSSWNLPPG PFPLPIIGNL FQLELKNIPK SFTRLAQRFG PVFTLYVGSQ RMVVMHGYKA VKEALLDYKD EFSGRGDLPA FHAHRDRGII FNNGPTWKDI RRFSLTTLRN YGMGKQGNES RIQREAHFLL EALRKTQGQP FDPTFLIGCA PCNVIADILF RKHFDYNDEK FLRLMYLFNE NFHLLSTPWL QLYNNFPSFL HYLPGSHRKV IKNVAEVKEY VSERVKEHHQ SLDPNCPRDL TDCLLVEMEK EKHSAERLYT MDGITVTVAD LFFAGTETTS TTLRYGLLIL MKYPEIEEKL HEEIDRVIGP SRIPAIKDRQ EMPYMDAVVH EIQRFITLVP SNLPHEATRD TIFRGYLIPK GTVVVPTLDS VLYDNQEFPD PEKFKPEHFL NENGKFKYSD YFKPFSTGKR VCAGEGLARM ELFLLLCAIL QHFNLKPLVD PKDIDLSPIH IGFGCIPPRY KLCVIPRS. |
| Specifications |
After Desmin is dissolved in 9.5M urea buffer (see formulation), protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50mM NaCl, 2mM dithiothreitol, 10mM Tris-HCl, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS). |
