CYP2E1 Human

Data sheet

Formulation

CYP2E1 protein solution (0.5mg/ml) containing 20mM Tris-HCl (pH8.0) and 10% glycerol.

Purity

Greater than 85.0% as determined by SDS-PAGE.

Description

CYP2E1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 488 amino acids (29-493 a.a) and having a molecular mass of 56.2kDa. CYP2E1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary

Protein Background

CYP2E1, also known as Cytochrome P450 2E1 belongs to the cytochrome P450 mixed-function oxidase system which is involved in the metabolism of xenobiotics in the body. While CYP2E1 is implicated in the oxidative metabolism of small range substrates, commo

Expression host

Escherichia Coli.

Synonyms

Cytochrome P450, Family 2, Subfamily E, Polypeptide 1, CYP2E, Cytochrome P450, Subfamily IIE (Ethanol-Inducible), Polypeptide 1, 4-Nitrophenol 2-Hydroxylase, Cytochrome P450-J, P450C2E, CYPIIE1, Flavoprotein-Linked Monooxygenase, Microsomal Monooxygenase,

Reagent Appearance

Sterile filtered colorless solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSSWNLPPG PFPLPIIGNL FQLELKNIPK SFTRLAQRFG PVFTLYVGSQ RMVVMHGYKA VKEALLDYKD EFSGRGDLPA FHAHRDRGII FNNGPTWKDI RRFSLTTLRN YGMGKQGNES RIQREAHFLL EALRKTQGQP FDPTFLIGCA PCNVIADILF RKHFDYNDEK FLRLMYLFNE NFHLLSTPWL QLYNNFPSFL HYLPGSHRKV IK

Specifications

After Desmin is dissolved in 9.5M urea buffer (see formulation), protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50mM NaCl, 2mM dithi