| Formulation |
CXCL16 protein was lyophilized from a 0.2um filtered solution in PBS. |
| Solubility |
It is recommended to reconstitute the lyophilized CXCL16 in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
| Purity |
Greater than 97.0% as determined by SDS-PAGE. |
| Description |
CXCL16 Human Recombinant (30-118 a.a.) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 89 amino acids and having a molecular mass of 10kDa.The CXCL16 is purified by proprietary chromatographic techniques. |
| Protein Background |
CXC chemokine ligand 16 (CXCL16) is a type I membrane protein, which contains a non-ELR motif-containing CXC chemokine domain in its extracellular region. Human and Mouse CXCL16 share a 70% a.a. sequence similarity within their chemokine domains and 49% overall a.a. sequence identity. Functional CXCL16 can be dropped from the cell surface as an approximately 35kDa soluble protein. CXCR6 is the functional receptor for CXCL16. CXCL16 functions as a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), proposing that CXCL16 may be involved in pathophysiology such as atherogenesis. In addition, CXCL16 induces a strong chemotactic response and calcium mobilization. CXCL16 binds to CXCR6/Bonzo. |
| Expression host |
Escherichia Coli. |
| Synonyms |
Chemokine (C-X-C Motif) Ligand 16, Scavenger Receptor For Phosphatidylserine And Oxidized Low Density Lipoprotein, Transmembrane Chemokine CXCL16, Small-Inducible Cytokine B16, CXC Chemokine Ligand 16, SR-PSOX, SRPSOX, C-X-C Motif Chemokine 16, CXCLG16, SCYB16, CXCL16. |
| Reagent Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Stability |
Lyophilized CXCL16 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CXCL16 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Amino acid sequence |
NEGSVTGSCY CGKRISSDSP PSVQFMNRLR KHLRAYHRCL YYTRFQLLSW SVCGGNKDPW VQELMSCLDL KECGHAYSGI VAHQKHLLP. |
| Biological Activity |
The ED50, as measured by its ability to chemoattract mouse CXCR6-transfected mouse BaF3 cells, is less than 12ng/ml. |
| Assay Conditions |
1. Prepare 526-532nm colloid gold, conjugation within 24 hours after colloid gold preparation.2. Adjust colloid gold OD at 1.3-1.4, conjugation concentration and pH are 16ug/ml gold and 7.2.3. Shanking conjugation for 8-10 hours, then centrifuge the conjugation at 10,000 rpm at 20oC for 20 min.4. Remove the supernatnant as more as possible, add the supplied gold suspension buffer to adjust OD to 8.2.5. Add the supplied blocking reagent to the final concentration to 0.8%.6. Blocking for 1 hour, then add sucrose to the final concentration to 10 to 12%.7. Dipping the conjugated gold to the gold pad (do not use sodium phosphate for gold pad treatment).8. After drying, assemble gold pad, the sample padand absorbent pad over backing card. 9. Add 10ul serum/plasma and add the supplied epi-10 running buffer for assay. |
