CST3 Human, Active

Data sheet

Formulation

The Cystatin-C was lyophilized from 1mg/ml in 1xPBS.

Solubility

It is recommended to reconstitute the lyophilized Cystatin-C in sterile water not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 95% as obsereved by SDS-PAGE.

Description

Cystatin-C Human Recombinant produced in HEK cells is a non-glycosylated monomer, having a molecular weight of approximately 13kDa.The Cystatin-C is purified by proprietary chromatographic techniques.

Protein Background

Cystatins are a superfamily of cysteine proteinase inhibitors found in both plants and animals. They comprise a group of proteinase inhibitors, widely distributed in tissues and body fluids, and form tight complexes with cysteine proteases such as catheps

Expression host

HEK.

Synonyms

Cystatin-C, Cystatin-3, Neuroendocrine basic polypeptide, Gamma-trace, Post-gamma-globulin, CST3, MGC117328.

Stability

Lyophilized Cystatin-C although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Cystatin-C should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended

Physical Appearence

Sterile Filtered White lyophilized (freeze-dried) powder.

Biological Activity

The inhibitory function of Cystatin-C on papins protease activity was measured by a colorimetric assay using L-BAPA as substrate. IC50 value was measured at 5-20 ug/ml (0.3-1.5 uM) with a range of 1.56-50ug/ml Cystatin-C in presence of 0.55uM papain and 0