| Formulation |
CRISP2 filtered (0.4um) solution at a concentration of 0.3mg/ml in 50mM acetate buffer, pH 4, 5mM DTT and 20%glycerol. |
| Purity |
Greater than 90.0% as determined by SDS-PAGE. |
| Description |
CRISP2 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (22-243) containing 232 amino acids including a 10 aa His tag at N-terminus. The total calculated molecular mass is 26.32kDa. |
| Protein Background |
Cysteine-Rich Secretory Protein 2 (CRISP2) regulates some ion channels activity and thereby regulates calcium fluxes during sperm capacitation. Amongst its related pathways are p53 signaling. Diseases related with CRISP2 include epididymitis and orchitis. |
| Expression host |
Escherichia Coli. |
| Synonyms |
Cysteine-Rich Secretory Protein 2, Cancer/Testis Antigen 36, TPX1, Testis Specific Protein 1 (Probe H4-1 P3-1), Testis-Specific Protein TPX-1, CRISP-2, GAPDL5, TSP1, CT36, Glyceraldehyde-3-Phosphate Dehydrogenase-Like 5, CRISP2. |
| Reagent Appearance |
Filtered colorless solution. |
| Stability |
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Amino acid sequence |
MKHHHHHHASKDPAFTALLT TQLQVQREIV NKHNELRKAV SPPASNMLKM EWSREVTTNA QRWANKCTLQ HSDPEDRKTS TRCGENLYMS SDPTSWSSAI QSWYDEILDF VYGVGPKSPN AVVGHYTQLV WYSTYQVGCG IAYCPNQDSL KYYYVCQYCP AGNNMNRKNT PYQQGTPCAG CPDDCDKGLC TNSCQYQDLL SNCDSLKNTA GCEHELLKEK CKATCLCENK IY. |
