CRIPT Human

Data sheet

Formulation

The CRIPT solution (1mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 100mM NaCl, 1mM DTT and 20% glycerol.

Purity

Greater than 95% as determined by SDS-PAGE.

Description

CRIPT Human Recombinant produced in E. coli is a single polypeptide chain containing 124 amino acids (1-101) and having a molecular mass of 13.7kDa.CRIPT is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic technique

Protein Background

Cysteine-rich PDZ-binding protein, also known as CRIPT, takes part in the cytoskeletal anchoring of DLG4 in excitatory synapses.

Expression host

E.coli.

Synonyms

Cysteine-rich interactor of PDZ three, cysteine-rich PDZ-binding protein, Cysteine-rich interactor of PDZ3, postsynaptic protein CRIPT, HSPC139.

Reagent Appearance

Sterile Filtered colorless solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Amino acid sequence

MGSIHHHHHH SSGLVPRGSH MGSMVCEKCE KKLGTVITPD TWKDGARNTT ESGGRKLNEN KALTSKKARF DPYGKNKFST CRICKSSVHQ PGSHYCQGCA YKKGICAMCG KKVLDTKNYK QTSV