| Clone |
PAT14G8AT. |
| Applications |
CRADD antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:500. |
| Protein Background |
CRADD is a 22kDa, widely-expressed cytosolic adaptor/signaling protein that induces cell apoptosis/cell death in numerous tissues. CRADD is a death domain (CARD) that recruits, caspase 2/ICH1 to the cell death signal transduction complex that includes TNFR1A, RIPK1/RIP kinase, and numbers of other CARD domain-containing proteins. |
| Synonyms |
RAIDD, MGC9163, CRADD, Death domain-containing protein CRADD, Caspase and RIP adapter with death domain, RIP-associated protein with a death domain, CASP2 and RIPK1 domain containing adaptor with death domain. |
| Reagent Appearance |
Sterile filtered colorless solution. |
| Type |
Mouse Anti Human Monoclonal. |
| Immunogen |
Anti-human CRADD mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human CRADD protein 1-199 amino acids purified from E. coli. |
| Ig Subclass |
Mouse IgG1 heavy chain and k light chain. |
| Purification Method |
CRADD antibody was purified from mouse ascitic fluids by protein-A affinity chromatography. |
| Protein formulation |
1mg/ml containing PBS, pH-7.4, 10% Glycerol and 0.02% Sodium Azide. |
| Storage Procedures |
For periods up to 1 month store at 4°C, for longer periods of time, store at -20°C. Prevent freeze thaw cycles. |
| Stability / Shelf Life |
12 months at -20°C. 1 month at 4°C. |
