CKMT2 Human

Data sheet

Formulation

The CKMT2 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, 1mM DTT and 10% glycerol.

Purity

Greater than 90% as determined by SDS-PAGE.

Description

CKMT2 Human Recombinant produced in E. coli is a single polypeptide chain containing 405 amino acids (40-419) and having a molecular mass of 46.1 kDa.CKMT2 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniq

Protein Background

Creatine Kinase, Mitochondrial 2 (CKMT2) is a member of the ATP:guanido phosphotransferase family. CKMT2 is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. CKMT2 reversibly catalyzes the transfer

Expression host

E.coli.

Synonyms

Creatine kinase mitochondrial 2 (sarcomeric), Basic-type mitochondrial creatine kinase, Sarcomeric mitochondrial creatine kinase, creatine kinase S-type, mitochondrial, SMTCK, Mib-CK, EC 2.7.3.2.

Reagent Appearance

Sterile Filtered colorless solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSHMEVREQ PRLFPPSADY PDLRKHNNCM AECLTPAIYA KLRNKVTPNG YTLDQCIQTG VDNPGHPFIK TVGMVAGDEE SYEVFADLFD PVIKLRHNGY DPRVMKHTTD LDASKITQGQ FDEHYVLSSR VRTGRSIRGL SLPPACTRAE RREVENVAIT ALEGLKGDLA GRYYKLSEMT EQDQQRLIDD HFLFDKPVSP LLTCAGMARD WP