| Formulation |
The CHAC2 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 100mM NaCl and 10% glycerol. |
| Purity |
Greater than 90% as determined by SDS-PAGE. |
| Description |
CHAC2 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 207 amino acids (1-184) and having a molecular mass of 23.3 kDa. CHAC2 is fused to a 23 amino acid His-tag at N-terminus. |
| Protein Background |
Cation transport regulator-like protein 2 (CHAC2) is a member of the chaC family. Catalyzes the cleavage glutathione into 5-oxoproline and a Cys-Gly dipeptide. Acts specifically on glutathione, but not on other gamma-glutamyl peptides. |
| Expression host |
Escherichia Coli. |
| Synonyms |
ChaC Cation Transport Regulator Homolog 2 (E. Coli), ChaC Cation Transport Regulator-Like 2 (E. Coli), Gamma-GCT Acting On Glutathione Homolog 2. |
| Reagent Appearance |
Sterile Filtered clear solution. |
| Stability |
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Amino acid sequence |
MGSSHHHHHH SSGLVPRGSH MGSMWVFGYG SLIWKVDFPY QDKLVGYITN YSRRFWQGST DHRGVPGKPG RVVTLVEDPA GCVWGVAYRL PVGKEEEVKA YLDFREKGGY RTTTVIFYPK DPTTKPFSVL LYIGTCDNPD YLGPAPLEDI AEQIFNAAGP SGRNTEYLFE LANSIRNLVP EEADEHLFAL EKLVKERLEG KQNLNCI |
