CEL Human

Data sheet

Formulation

CEL protein filtered (0.4um) and lyophilized in 0.5mg/ml in 0.05M phosphate buffer and 0.075M NaCl pH 7.4.

Solubility

It is recommended to add deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. CEL is not sterile! Please filter the product by an appropriate sterile filter before using it in t

Purity

Greater than 95.0% as determined by SDS-PAGE.

Description

The Carboxyl Ester Lipase produced from Human breast milk has a molecular mass of 79.322kDa (calculated without glycosylation) containing 733 amino acid residues.

Protein Background

Bile salt-activated lipase (BAL or CEL) is a glycoprotein secreted from the pancreas into the digestive tract and from the lactating mammary gland into human milk. The milk of mammals, including humans, contains BAL to enable fat absorption in infants. Th

Expression host

Human breast milk.

Synonyms

Bile salt-activated lipase, BAL, EC 3.1.1.13, EC 3.1.1.3, Bile salt-stimulated lipase, BSSL, Bucelipase, Carboxyl ester lipase, Cholesterol esterase, Pancreatic lysophospholipase, Sterol esterase, CEL, FAP, BSDL, CELL, FAPP, LIPA, Cease, MODY8.

Reagent Appearance

Filtered White lyophilized (freeze-dried) powder.

Stability

Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

Amino acid sequence

AKLGAVYTEG GFVEGVNKKL GLLGDSVDIF KGIPFAAPTK ALENPQPHPG WQGTLKAKNF KKRCLQATIT QDSTYGDEDC LYLNIWVPQG RKQVSRDLPV MIWIYGGAFL MGSGHGANFL NNYLYDGEEI ATRGNVIVVT FNYRVGPLGF LSTGDANLPG NYGLRDQHMA IAWVKRNIAA FGGDPNNITL FGESAGGASV SLQTLSPYNK GLIRRAISQS GVALSPWVIQ KN