CDNF Human

Data sheet

Formulation

CDNF protein was lyophilized from a 0.2um filtered concentrated solution in 1xPBS, pH 7.4.

Solubility

It is recommended to reconstitute the lyophilized CDNF in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 96.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.

Description

CDNF Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 161 amino acids and having a molecular mass of 18.5kDa.The CDNF is purified by proprietary chromatographic techniques.

Protein Background

CDNF is a member of the ARMET family and acts as a trophic factor for dopamine neurons. CDNF inhibits the 6-hydroxydopamine (6-OHDA)-induced degeneration of dopaminergic neurons. When CDNF controlled after 6-OHDA-lesioning, it reestablishes the dopaminerg

Expression host

Escherichia Coli.

Synonyms

Cerebral dopamine neurotrophic factor, arginine-rich, mutated in early stage tumors-like 1, Conserved dopamine neurotrophic factor, ARMET-like protein 1, ARMETL1.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized CDNF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CDNF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a c

Amino acid sequence

QEAGGRPGAD CEVCKEFLNR FYKSLIDRGV NFSLDTIEKE LISFCLDTKG KENRLCYYLG ATKDAATKIL SEVTRPMSVH MPAMKICEKL KKLDSQICEL KYEKTLDLAS VDLRKMRVAE LKQILHSWGE ECRACAEKTD YVNLIQELAP KYAATHPKTE L

Biological Activity

The ED50 as determined by its ability to stimulate the proliferation of rat C6 cells is 15-25ug/ml, corresponding to a specific activity of 40-67units/mg.