CDK2 Human, Sf9

Data sheet

Formulation

CDK2 protein solution (0. 5mg/ml) contains Phosphate buffered saline (pH7.4), 30% glycerol, 2mM DTT and 0.1mM PMSF.

Purity

Greater than 90.0% as determined by SDS-PAGE.

Description

CDK2 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 306 amino acids (1-298a.a.) and having a molecular mass of 34.9kDa. CDK2 is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chr

Protein Background

Cyclin-dependent kinase 2 (CDK2) belongs the Ser/Thr protein kinase family. CDK2 is highly parallel to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. CDK2 is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity

Expression host

Sf9, Baculovirus cells.

Synonyms

Cyclin-Dependent Kinase 2, Cell Division Protein Kinase 2, P33 Protein Kinase, EC 2.7.11.22, CDKN2, Cdc2-Related Protein Kinase, P33(CDK2), EC 2.7.11, Cyclin-dependent kinase 2.

Reagent Appearance

Sterile filtered colorless solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Amino acid sequence

MENFQKVEKI GEGTYGVVYK ARNKLTGEVV ALKKIRLDTE TEGVPSTAIR EISLLKELNH PNIVKLLDVI HTENKLYLVF EFLHQDLKKF MDASALTGIP LPLIKSYLFQ LLQGLAFCHS HRVLHRDLKP QNLLINTEGA IKLADFGLAR AFGVPVRTYT HEVVTLWYRA PEILLGCKYY STAVDIWSLG CIFAEMVTRR ALFPGDSEID QLFRIFRTLG TPDEVVWPGV T