| Formulation |
CDK1 protein solution (0. 5mg/ml) contains Phosphate buffered saline (pH7.4), 30% glycerol, 2mM DTT and 0.1mM PMSF. |
| Purity |
Greater than 95.0% as determined by SDS-PAGE. |
| Description |
CDK1 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 305 amino acids (1-297a.a.) and having a molecular mass of 35.1kDa. CDK1 is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques. |
| Protein Background |
Cyclin-dependent kinase 1 (CDK1) plays a significant part in the control of the eukaryotic cell cycle through modulating the centrosome cycle in addition to mitotic onset; CDK1 promotes G2-M transition and regulates G1 progress and G1-S transition using association with multiple interphase cyclins. CDK1 is essential in higher cells for the entry into S-phase and mitosis. |
| Expression host |
Sf9, Baculovirus cells. |
| Synonyms |
Cyclin-Dependent Kinase 1, CDC2, Cell Division Cycle 2, G1 To S And G2 To M, Cell Division Control Protein 2 Homolog, Cell Division Protein Kinase 1, P34 Protein Kinase, P34CDC2, CDC28A, Cell Cycle Controller CDC2, EC 2.7.11.22, EC 2.7.11.23, CDKN1, Cyclin-dependent kinase 1. |
| Reagent Appearance |
Sterile filtered colorless solution. |
| Stability |
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Amino acid sequence |
MEDYTKIEKI GEGTYGVVYK GRHKTTGQVV AMKKIRLESE EEGVPSTAIR EISLLKELRH PNIVSLQDVL MQDSRLYLIF EFLSMDLKKY LDSIPPGQYM DSSLVKSYLY QILQGIVFCH SRRVLHRDLK PQNLLIDDKG TIKLADFGLA RAFGIPIRVY THEVVTLWYR SPEVLLGSAR YSTPVDIWSI GTIFAELATK KPLFHGDSEI DQLFRIFRAL GTPNNEVWPE VESLQDYKNT FPKWKPGSLA SHVKNLDENG LDLLSKMLIY DPAKRISGKM ALNHPYFNDL DNQIKKMLEH HHHHH. |
| Product Description |
Immunoreactive with sera of Chlamydia Trachomatis infected individuals with minimum specificity problems. |
| Purity Method |
The E.coli derived recombinant 6xHis fusion at C-terminus protein contains Chlamydia TrachomatisMOMP protein epitopes, 252-398 amino acids. |
