| Formulation |
CD43 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl and 10% glycerol. |
| Purity |
Greater than 85% as determined by SDS-PAGE. |
| Description |
CD43 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 257 amino acids (20-253a.a.) and having a molecular mass of 25.8kDa.CD43 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
| Protein Background |
CD43 is a key sialoglycoprotein located in the surface of T lymphocytes, monocytes, granulocytes, thymocytes and some B lymphocytes. CD43 is a part of a physiologic ligand-receptor complex which participates in T-cell activation. Throughout T-cell activation, CD43 is actively taken out from the T-cell-APC (antigen-presenting cell) contact site, signifying CD43 has a negative regulatory role in adaptive immune response. |
| Expression host |
E.coli. |
| Synonyms |
Leukosialin, Galactoglycoprotein, GALGP, Leukocyte sialoglycoprotein, Sialophorin, SPN, CD43, leukosialin precursor, GPL115, LSN. |
| Reagent Appearance |
Sterile Filtered colorless solution. |
| Stability |
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Amino acid sequence |
MGSSHHHHHH SSGLVPRGSH MGSSTTAVQT PTSGEPLVST SEPLSSKMYT TSITSDPKAD STGDQTSALP PSTSINEGSP LWTSIGASTG SPLPEPTTYQ EVSIKMSSVP QETPHATSHP AVPITANSLG SHTVTGGTIT TNSPETSSRT SGAPVTTAAS SLETSRGTSG PPLTMATVSL ETSKGTSGPP VTMATDSLET STGTTGPPVT MTTGSLEPSS GASGPQVSSV KLSTMMSPTT STNASTVPFR NPDENSR. |
