| Formulation |
Recombinant Human protein at 100ug/ml in 50mM Tris-HCl, (pH-7.5) and 10mM L-glutathione. |
| Applications |
ELISA. Inhibition Assays. Western Blotting. |
| Description |
CD-4 Human Recombinant encoding amino acids 26-226. CD4 is fused to a GST tag at N-terminus and purified by standard chromatography techniques. |
| Protein Background |
CD4, a single chain transmembrane glycoprotein, is found on a T cell subset (helper/inducer) representing 45% of peripheral blood lymphocytes. It is also present on 80% of thymocytes and at a lower level on monocytes. It is involved in recognition of antigen presented along with MHC class II by APCs. It serves as receptor for HIV. Antibody to CD4 recognizes T-helper cells required for recognition of class II antigens. It reacts with ~60% of peripheral blood E rosette-positive (E+) cells while showing negligible reactivity with E- cells, monocytes, granulocytes, EBV-transformed B cell lines, and mouse splenocytes. |
| Expression host |
Escherichia Coli. |
| Synonyms |
gp55, HLA-2, L3 / T4, Ly-4, T cell antigen T4/LEU3, T4, sCD4. |
| Reagent Appearance |
Sterile filtered liquid. |
| Stability |
Store vial at -20oC to -80oC. When stored at the recommended temperature, this protein is stable for 12 months. Please avoid freeze-thaw cycles. |
| Characterization |
On SDS-PAGE commassie blue stained gel, the purified recombinant protein shows a band at 48kDa. |
