CD34 Human, Sf9

Data sheet

Reconstitution

1mg/ml in PBS (after reconstitution).

Formulation

CD34 protein solution (0.25mg/ml) contains Phosphate buffered saline (pH7.4) and 10% glycerol.

Purity

Greater than 95.0% as determined by SDS-PAGE.

Description

CD34 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 268 amino acids (32-290a.a.) and having a molecular mass of 28.6kDa (Molecular size on SDS-PAGE will appear at approximately 40-57kDa).CD34 is

Protein Background

CD34 is a single chain transmembrane glycoprotein which is selectively expressed on human lymphoid and myeloid hemapoietic progenitor cells. CD34 has been used to measure angiogenesis, which reportedly predicts tumor recurrence.

Expression host

Sf9, Baculovirus cells.

Synonyms

CD34 molecule, Hematopoietic progenitor cell antigen CD34, CD34 Antigen, CD34.

Reagent Appearance

Sterile filtered colorless solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Amino acid sequence

ADLSLDNNGT ATPELPTQGT FSNVSTNVSY QETTTPSTLG STSLHPVSQH GNEATTNITE TTVKFTSTSV ITSVYGNTNS SVQSQTSVIS TVFTTPANVS TPETTLKPSL SPGNVSDLST TSTSLATSPT KPYTSSSPIL SDIKAEIKCS GIREVKLTQG ICLEQNKTSS CAEFKKDRGE GLARVLCGEE QADADAGAQV CSLLLAQSEV RPQCLLLVLA NRTEISSKLQ LM

Antibody Properties

Reconstitute with of H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.

Available Conjugates

Cytotoxic and staining antibody. For staining, use 10ul/1,000,000 cells. Exact titer for cytotoxicity should be determined by the investigator.

Immunological function

This antibody is only available as purified antibody.