CD33 Human

Data sheet

Reconstitution

1mg/ml in PBS (after reconstitution).

Formulation

CD33 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea, and 10% glycerol.

Purity

Greater than 90% as determined by SDS-PAGE.

Description

CD33 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 265 amino acids (18-259 a.a) and having a molecular mass of 29.1kDa.CD33 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chrom

Protein Background

CD33 is putative adhesion molecule of myelomonocytic-derived cells which mediates sialic-acid dependent binding to cells. CD33 prefers to bind to alpha-2,6-linked sialic acid. The sialic acid recognition site is masked by cis interactions with sialic acid

Expression host

Escherichia Coli.

Synonyms

CD33Molecule, CD33Antigen (Gp67), Sialic Acid Binding Ig-Like Lectin 3, Sialic Acid-Binding Ig-Like Lectin 3, SIGLEC3, gp67, SIGLEC-3, Myeloid Cell Surface AntigenCD33, p67, Siglec-3, CD33Antigen.

Reagent Appearance

Sterile Filtered clear solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze thaw cycles.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSDPNFWLQ VQESVTVQEG LCVLVPCTFF HPIPYYDKNS PVHGYWFREG AIISGDSPVA TNKLDQEVQE ETQGRFRLLG DPSRNNCSLS IVDARRRDNG SYFFRMERGS TKYSYKSPQL SVHVTDLTHR PKILIPGTLE PGHSKNLTCS VSWACEQGTP PIFSWLSAAP TSLGPRTTHS SVLIITPRPQ DHGTNLTCQV KFAGAGVTTE RT

Antibody Properties

Reconstitute with of H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.

Available Conjugates

FACS staining, immunohistochemistry, Western Blotting. For staining, use 10 ul/1,000,000 cells.

Immunological function

This antibody is only available unconjugated and conjugated to Biotin.