| Reconstitution |
1mg/ml in PBS (after reconstitution). |
| Formulation |
CD300C protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.4M urea and 10% glycerol. |
| Purity |
Greater than 85.0% as determined by SDS-PAGE. |
| Description |
CD300C Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 186 amino acids (21-183a.a.) and having a molecular mass of 20.2kDa. CD300C is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
| Protein Background |
CMRF35-like molecule 6 (CD300C), is a member of the CD300 family. The CMRF35 antigen was identified by reactivity with a monoclonal antibody and present on monocytes, neutrophils, and some T and B lymphocytes. |
| Expression host |
Escherichia Coli. |
| Synonyms |
CLM-6, CMRF-35, CMRF-35A, CMRF35, CMRF35-A1, CMRF35A, CMRF35A1, IGSF16, LIR, CMRF35-like molecule 6, CD300 antigen-like family member C, Immunoglobulin superfamily member 16. |
| Reagent Appearance |
Sterile Filtered clear solution. |
| Stability |
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Amino acid sequence |
MGSSHHHHHH SSGLVPRGSH MGSGYFPLSH PMTVAGPVGG SLSVQCRYEK EHRTLNKFWC RPPQILRCDK IVETKGSAGK RNGRVSIRDS PANLSFTVTL ENLTEEDAGT YWCGVDTPWL RDFHDPIVEV EVSVFPAGTT TASSPQSSMG TSGPPTKLPV HTWPSVTRKD SPEPSPHPGSLFSNVR. |
| Antibody Properties |
Reconstitute with of H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. |
| Available Conjugates |
Blocking and staining antibody. For staining, use 10ul/1,000,000 cells. Titer for blocking T cell activation should be determined by the investigator. |
| Immunological function |
This antibody is only available non-conjugated and conjugated to FITC. |
