| Reconstitution |
1mg/ml in PBS (after reconstitution). |
| Formulation |
CD1E protein solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 10% glycerol and 0.4M Urea. |
| Purity |
Greater than 85.0% as determined by SDS-PAGE. |
| Description |
CD1E Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 297 amino acids (32-305 a.a) and having a molecular mass of 33.1kDa.CD1E is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
| Protein Background |
CD1E belongs to the CD1 family of transmembrane glycoproteins which are structurally associated to the main MHC (histocompatibility complex) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome is comprised of 5 CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are belief to differ in their cellular localization and specificity for particular lipid ligands. CD1E localizes within Golgi compartments, endosomes, and lysosomes, and is cleaved into a steady soluble form. The soluble form is necessary for the intracellular processing of some glycolipids into a form that can be presented by other CD1 family members. |
| Expression host |
Escherichia Coli. |
| Synonyms |
CD1A, R2, T-cell surface glycoprotein CD1e, membrane-associated, R2G1, sCD1e. |
| Reagent Appearance |
Sterile Filtered clear solution. |
| Stability |
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Amino acid sequence |
MGSSHHHHHH SSGLVPRGSH MGSEEQLSFR MLQTSSFANH SWAHSEGSGW LGDLQTHGWD TVLGTIRFLK PWSHGNFSKQ ELKNLQSLFQ LYFHSFIQIV QASAGQFQLE YPFEIQILAG CRMNAPQIFL NMAYQGSDFL SFQGISWEPS PGAGIRAQNI CKVLNRYLDI KEILQSLLGH TCPRFLAGLM EAGESELKRK VKPEAWLSCG PSPGPGRLQL VCHVSGFYPK PVWVMWMRGE QEQRGTQRGD VLPNADETWY LRATLDVAAG EAAGLSCRVK HSSLGGHDLI IHWGGYS. |
| Antibody Properties |
Reconstitute with of H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. |
| Available Conjugates |
Staining antibody. For staining, use 10ul/1,000,000 cells. |
| Immunological function |
This antibody is also available conjugated to biotin and FITC. For staining with biotin or FITC-conjugated antibody use 5-10ul/1,000,000 cells. |
