CD1E Human

Data sheet

Reconstitution

1mg/ml in PBS (after reconstitution).

Formulation

CD1E protein solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 10% glycerol and 0.4M Urea.

Purity

Greater than 85.0% as determined by SDS-PAGE.

Description

CD1E Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 297 amino acids (32-305 a.a) and having a molecular mass of 33.1kDa.CD1E is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chrom

Protein Background

CD1E belongs to the CD1 family of transmembrane glycoproteins which are structurally associated to the main MHC (histocompatibility complex) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily l

Expression host

Escherichia Coli.

Synonyms

CD1A, R2, T-cell surface glycoprotein CD1e, membrane-associated, R2G1, sCD1e.

Reagent Appearance

Sterile Filtered clear solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSEEQLSFR MLQTSSFANH SWAHSEGSGW LGDLQTHGWD TVLGTIRFLK PWSHGNFSKQ ELKNLQSLFQ LYFHSFIQIV QASAGQFQLE YPFEIQILAG CRMNAPQIFL NMAYQGSDFL SFQGISWEPS PGAGIRAQNI CKVLNRYLDI KEILQSLLGH TCPRFLAGLM EAGESELKRK VKPEAWLSCG PSPGPGRLQL VCHVSGFYPK PV

Antibody Properties

Reconstitute with of H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.

Available Conjugates

Staining antibody. For staining, use 10ul/1,000,000 cells.

Immunological function

This antibody is also available conjugated to biotin and FITC. For staining with biotin or FITC-conjugated antibody use 5-10ul/1,000,000 cells.