CAT Human

Data sheet

Reconstitution

1mg/ml in PBS (after reconstitution).

Formulation

The CAT solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl and 10% glycerol.

Purity

Greater than 90.0% as determined by SDS-PAGE.

Description

CAT Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 547 amino acids (1-527) and having a molecular mass of 61.9kDa.CAT is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatograp

Protein Background

Catalase (CAT) is a key antioxidant enzyme in the bodys defense against oxidative stress. Furthermore, Catalase is a heme enzyme which is present in the peroxisome of virtually all aerobic cells. Catalase converts the reactive oxygen species hydrogen pero

Expression host

Escherichia Coli.

Synonyms

Catalase, CAT.

Reagent Appearance

Sterile filtered yellowish solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MADSRDPASD QMQHWKEQRA AQKADVLTTG AGNPVGDKLN VITVGPRGPL LVQDVVFTDE MAHFDRERIP ERVVHAKGAG AFGYFEVTHD ITKYSKAKVF EHIGKKTPIA VRFSTVAGES GSADTVRDPR GFAVKFYTED GNWDLVGNNT PIFFIRDPIL FPSFIHSQKR NPQTHLKDPD MVWDFWSLRP ESLHQVSFLF SDRGIPDGHR HM

Unit Definition

One unit will decompose 1.0 umole of H2O2 per minute at pH 8.0 at 25°C.

Biological Activity

Specific activity is >30,000 unit/mg.

Antibody Properties

Reconstitute with of H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.

Available Conjugates

Staining antibody. For staining, use 10ul/1,000,000 cells. Titer for blocking/activating T cells should be determined by the investigator.