CASP2 Human

Data sheet

Reconstitution

1mg/ml in PBS (after reconstitution).

Formulation

The CASP2 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol.

Purity

Greater than 80.0% as determined by SDS-PAGE.

Description

CASP2 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 126 amino acids (348-452) and having a molecular mass of 14.1kDa.CASP2 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chroma

Protein Background

Caspase 2 Apoptosis-Related Cysteine Peptidase (CASP2) is a part of the cysteine-aspartic acid protease (caspase) family. CASP2 takes part in the activation cascade of caspases responsible for apoptosis execution. CASP2 is also responsible for inactivatin

Expression host

Escherichia Coli.

Synonyms

Caspase-2, CASP-2, CASP2, Caspase 2 Apoptosis-Related Cysteine Peptidase, Neural precursor cell expressed developmentally down-regulated protein 2, NEDD-2, Protease ICH-1, ICH1, NEDD2, Caspase-2 subunit p18, Caspase-2 subunit p13, Caspase-2 subunit p12, C

Reagent Appearance

Sterile Filtered colorless solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MAGKEKLPKM RLPTRSDMIC GYACLKGTAA MRNTKRGSWY IEALAQVFSE RACDMHVADM LVKVNALIKD REGYAPGTEF HRCKEMSEYC STLCRHLYLF PGHPPT.

Antibody Properties

Reconstitute with of H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.

Available Conjugates

Blocking and staining antibody. For staining, use 10ul/1,000,000 cells. Titer for blocking T cell activation should be determined by the investigator.

Immunological function

This antibody is only available conjugated to Biotin and FITC.