Formulation |
Car b 1.0109 is supplied in 20mM HEPES buffer pH-7.6, 250mM NaCl and 20% glycerol. |
Purity |
Greater than 80.0% as determined by SDS-PAGE. |
Description |
Recombinant Pollen allergen Car b 1 isoform produced in E.coli is a non-glycosylated, polypeptide chain having a calculated molecular mass of 18,757 Dalton. Car b 1.0109 is expressed with a 10xHis tag at N-terminus and purified by proprietary chromatographic techniques. |
Protein Background |
Pollen allergen Car b 1 isoform (Car b 1.0109) causes an allergic reaction in humans. |
Expression host |
Escherichia Coli. |
Synonyms |
Pollen allergen Car b 1 isoform, Car b 1, Car b 1.0109. |
Reagent Appearance |
Sterile Filtered clear solution. |
Stability |
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles. |
Immunological functions |
1. Binds IgE type human antibodies. 2. Immunodot test with positive/negative sera panels. |
Molar extinction coefficient |
8940; A280(1mg/ml)=0.477 |