| Formulation |
The BRK1 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 150mM NaCl and 20% glycerol. |
| Purity |
Greater than 90% as determined by SDS-PAGE. |
| Description |
BRK1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 98 amino acids (1-75) and having a molecular mass of 11.0 kDa. BRK1 is fused to a 23 amino acid His-tag at N-terminus. |
| Protein Background |
BRK1 is a member of the BRK1 family. BRK1 takes part in regulating actin and microtubule organization and is a fragment of a WAVE complex which activates the Arp2/3 complex. |
| Expression host |
Escherichia Coli. |
| Synonyms |
BRICK1 SCAR/WAVE Actin-Nucleating Complex Subunit, Haematopoietic Stem/Progenitor Cell Protein 300, Homolog (Arabidopsis Thaliana), Chromosome 3 Open Reading Frame 10, Probable Protein BRICK1, BRICK1, HSPC300, MDS027, C3orf10. |
| Reagent Appearance |
Sterile Filtered clear solution. |
| Stability |
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Amino acid sequence |
MGSSHHHHHH SSGLVPRGSH MGSMAGQEDP VQREIHQDWA NREYIEIITS SIKKIADFLN SFDMSCRSRL ATLNEKLTAL ERRIEYIEAR VTKGETLT |
