| Formulation |
The BMP6 solution (0.25mg/ml) contains 10mM Sodium citrate buffer (pH 3.5) and 10% glycerol. |
| Purity |
Greater than 85.0% as determined by SDS-PAGE. |
| Description |
BMP6 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 164 amino acids (375-513) and having a molecular mass of 18kDa.BMP6 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
| Protein Background |
The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules, which can induce ectopic bone growth. Various BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were initially identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Based upon its expression early in embryogenesis, BMP6 has a suggested role in early development. Moreover, the fact that the BMP6 is closely related to BMP5 and BMP7 leads to an assumption of possible bone inductive activity. |
| Expression host |
Escherichia Coli. |
| Synonyms |
Bone morphogenetic protein 6, BMP-6, VG-1-related protein, VG-1-R, VGR-1, BMP6, VGR, VGR1. |
| Reagent Appearance |
Sterile filtered colorless solution. |
| Stability |
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles. |
| Amino acid sequence |
MGSSHHHHHH SSGLVPRGSH MGSHMSASSR RRQQSRNRST QSQDVARVSS ASDYNSSELK TACRKHELYV SFQDLGWQDW IIAPKGYAAN YCDGECSFPL NAHMNATNHA IVQTLVHLMN PEYVPKPCCA PTKLNAISVL YFDDNSNVIL KKYRNMVVRA CGCH. |
| Quality Control Test |
MKHHHHHHAS RVPHVQGEQQ EWEGTEELPS PPDHAERAEE QHEKYRPSQD QGLPASRCLR CCDPGTSMYP ATAVPQINIT ILKGEKGDRG DRGLQGKYGK TGSAGARGHT GPKGQKGSMG APGERCKSHY AAFSVGRKKP MHSNHYYQTV IFDTEFVNLY DHFNMFTGKF YCYVPGLYFF SLNVHTWNQK ETYLHIMKNE EEVVILFAQV GDRSIMQSQS LMLELREQDQ VWVRLYKGER ENAIFSEELD TYITFSGYLVKHATEP. |
