BLOC1S5 Human

Data sheet

Formulation

BLOC1S5 protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, 40% glycerol, 2mM DTT, 0.1mM PMSF and 1mM EDTA.

Purity

Greater than 90.0% as determined by SDS-PAGE.

Description

BLOC1S5 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 210 amino acids (1-187 a.a) and having a molecular mass of 24kDa.BLOC1S5 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary ch

Protein Background

Muted homolog (MUTED) is a component of the BLOC-1 complex, a complex which is necessary for normal biogenesis of lysosome-related organelles (LRO), such as platelet dense granules and melanosomes. The BLOC-1 complex is needed to steer membrane protein ca

Expression host

Escherichia Coli.

Synonyms

Biogenesis of lysosome-related organelles complex 1 subunit 5, BLOC-1 subunit 5, Protein Muted homolog, BLOC1S5, MUTED.

Reagent Appearance

Sterile Filtered colorless solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSMSGGGTE TPVGCEAAPG GGSKKRDSLG TAGSAHLIIK DLGEIHSRLL DHRPVIQGET RYFVKEFEEK RGLREMRVLE NLKNMIHETN EHTLPKCRDT MRDSLSQVLQ RLQAANDSVC RLQQREQERK KIHSDHLVAS EKQHMLQWDN FMKEQPNKRA EVDEEHRKAM ERLKEQYAEM EKDLAKFSTF.

Advantages

Starting material donor has been tested and certified negative for antibodies to HIV-1, HIV-2, HCV, HBSAG.