| Formulation |
Bet v 2.0101 is supplied in 20mM HEPES buffer pH-7.6, 250mM NaCl and 20% glycerol. |
| Purity |
Greater than 80.0% as determined by SDS-PAGE. |
| Description |
Recombinant Profilin-1 produced in E.coli is a non-glycosylated, polypeptide chain having a calculated molecular mass of 15,625 Dalton. Bet v 2.0101 is expressed with a 10xHis tag at N-terminus and purified by proprietary chromatographic techniques. |
| Protein Background |
Profilin-1 (Bet v 2.0101) binds to actin and affects the structure of the cytoskeleton. At high concentrations, profiling-1 averts the polymerization of actin, whereas it augments it at low concentrations. By binding to PIP2, Profilin-1 inhibits the creation of IP3 and DG. |
| Expression host |
Escherichia Coli. |
| Synonyms |
Profilin-1, Allergen Bet v II, Pollen allergen Bet v 2, Bet v 2, BETVII, Bet v 2.0101. |
| Reagent Appearance |
Sterile Filtered clear solution. |
| Stability |
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Immunological functions |
1. Binds IgE type human antibodies. 2. Immunodot test with positive/negative sera panels. |
