| Formulation |
The B9D2 solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 50% glycerol, 2mM DTT and 1mM EDTA. |
| Purity |
Greater than 85% as determined by SDS-PAGE. |
| Description |
B9D2 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 198 amino acids (1-175) and having a molecular mass of 21.6 kDa.B9D2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
| Protein Background |
B9 Protein Domain 2 (B9D2) is a component of the tectonic-like complex which acts as a barrier that prevents diffusion of transmembrane proteins between the cilia and plasma membranes. B9D2 contains a B9 domain protein, which is found solely in ciliated organisms. B9D2 is upregulated throughout mucociliary differentiation. |
| Expression host |
E.coli. |
| Synonyms |
B9 Protein Domain 2, B9D2, B9 domain-containing protein 2, MKS1-related protein 2, MKSR2, ICIS-1, MKS10. |
| Reagent Appearance |
Sterile Filtered colorless solution. |
| Stability |
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Amino acid sequence |
MGSSHHHHHH SSGLVPRGSH MGSMAEVHVI GQIIGASGFS ESSLFCKWGI HTGAAWKLLS GVREGQTQVD TPQIGDMAYW SHPIDLHFAT KGLQGWPRLH FQVWSQDSFG RCQLAGYGFC HVPSSPGTHQ LACPTWRPLG SWREQLARAF VGGGPQLLHG DTIYSGADRY RLHTAAGGTV HLEIGLLLRN FDRYGVEC. |
