ATP6V1F Human

Data sheet

Formulation

ATP6V1F protein solution (0.5mg/ml) containing Phosphate buffered saline (pH7.4), 50% glycerol and 1mM DTT.

Purity

Greater than 90.0% as determined by SDS-PAGE.

Description

ATP6V1F Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 142 amino acids (1-119 a.a) and having a molecular mass of 15.8kDa.ATP6V1F is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary

Protein Background

ATPase Transporting, Lysosomal V1 Subunit F (ATP6V1F) is a component of vacuolar ATPase (V-ATPase); it is a multi-subunit enzyme, which mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is required f

Expression host

Escherichia Coli.

Synonyms

ATP6S14, VATF, Vma7, V-type proton ATPase subunit F, V-ATPase 14 kDa subunit.

Reagent Appearance

Sterile Filtered clear solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSMAGRGKL IAVIGDEDTV TGFLLGGIGE LNKNRHPNFL VVEKDTTINE IEDTFRQFLN RDDIGIILIN QYIAEMVRHA LDAHQQSIPA VLEIPSKEHP YDAAKDSILR RARGMFTAED LR.