ARFIP2 Human

Data sheet

Formulation

The ARFIP2 solution (0.25mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 1mM DTT and 40% glycerol.

Purity

Greater than 90% as determined by SDS-PAGE.

Description

ARFIP2 Human Recombinant produced in E. coli is a single polypeptide chain containing 364 amino acids (1-341) and having a molecular mass of 40.2 kDa.ARFIP2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techni

Protein Background

Arfaptin 2 (ARFIP2) is a Rac1 binding protein essential for Rac-mediated actin polymerization and the succeeding formation of membrane ruffles and lamellipodia. ARFIP2 is a putative target protein of ADP-ribosylation factor. ARFIP2 is also involved in mem

Expression host

E.coli.

Synonyms

ADP-ribosylation factor interacting protein 2, partner of RAC1 (arfaptin 2), Partner of RAC1, Protein POR1, arfaptin-2, POR1.

Reagent Appearance

Sterile Filtered colorless solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGS TDGILG KAATMEIPIH GNGEARQLPE DDGLEQDLQQ VMVSGPNLNE TSIVSGGYGG SGDGLIPTGS GRHPSHSTTP SGPGDEVARG IAGEKFDIVK KWGINTYKCT KQLLSERFGR GSRTVDLELE LQIELLRETK RKYESVLQLG RALTAHLYSL LQTQHALGDA FADLSQKSPE LQEEFGYNAE TQKLLCKNGE TLLGAVNFFV SS