| Clone |
PAT27E8A. |
| Applications |
The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1:5000. Recommended starting dilution is 1:1000. |
| Protein Background |
Acireductone dioxygenase 1 (ADI1) is a part of the acireductone dioxygenase family of metal-binding enzymes, which are involved in methionine salvage. ADI1 regulates mRNA processing in the nucleus, and carries out different functions depending on its localization. Related pseudogenes have been defined on chromosomes 8 and 20. ADI1 down-regulates cell migration arbitrated by MMP14. |
| Synonyms |
APL1, ARD, FLJ10913, HMFT1638, MTCBP-1, SIPL, 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase, Acireductone dioxygenase, Fe-ARD, Membrane-type 1 matrix metalloproteinase cytoplasmic tail-binding protein 1, Submergence-induced protein-like factor. |
| Reagent Appearance |
Sterile filtered colorless solution. |
| Type |
Mouse Anti Human Monoclonal. |
| Immunogen |
Anti-human ADI1 mAb, clone PAT27E8A, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human ADI1 protein 1-179 amino acids purified from E. coli. |
| Ig Subclass |
Mouse IgG1 heavy chain and Kappa light chain. |
| Protein formulation |
1mg/ml containing PBS, pH-7.4, 10% Glycerol and 0.02% Sodium Azide. |
| Storage Procedures |
For periods up to 1 month store at 4°C, for longer periods of time, store at -20°C. Prevent freeze thaw cycles. |
| Stability / Shelf Life |
12 months at -20°C. 1 month at 4°C. |
