ACAA2 Human

Data sheet

Formulation

The ACAA2 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT.

Purity

Greater than 85.0% as determined by SDS-PAGE.

Description

ACAA2 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 404 amino acids (17-397) and having a molecular mass of 42.6kDa.ACAA2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromat

Protein Background

Acetyl-COA Acyltransferase 2, (ACAA2) is a member of the thiolase family. ACAA2 catalyzes the final step of the mitochondrial fatty acid beta-oxidation spiral. Not like most mitochondrial matrix proteins, ACAA2 contains a non-cleavable amino-terminal targ

Expression host

Escherichia Coli.

Synonyms

DSAEC, 3-ketoacyl-CoA thiolase, mitochondrial, Acetyl-CoA acyltransferase, Beta-ketothiolase.

Reagent Appearance

Sterile Filtered clear solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSFGAYGGL LKDFTATDLS EFAAKAALSA GKVSPETVDS VIMGNVLQSS SDAIYLARHV GLRVGIPKET PALTINRLCG SGFQSIVNGC QEICVKEAEV VLCGGTESMS QAPYCVRNVR FGTKLGSDIK LEDSLWVSLT DQHVQLPMAM TAENLAVKHK ISREECDKYA LQSQQRWKAA NDAGYFNDEM APIEVKTKKG KQTMQVDEHA RP