Pfu-DNA Polymerase Recombinant ( Pfu DNA Polymerase ) (PT_41744)
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  • Pfu-DNA Polymerase Recombinant ( Pfu DNA Polymerase ) (PT_41744)

Pfu-DNA Polymerase Recombinant ( Pfu DNA Polymerase )

Catalog # PT_41744

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$292.00
DescriptionPfu DNA Polymerase is a thermo-stable enzyme having a Mw of about 90kDa. Pfu DNA Polymerase is derived from E. coli that and cloned from Pyrococcus furiosus strain Vc1 DSM3638. Pfu DNA Polymerase replicates DNA at 75°C, catalyzing the
Product Size: 100U
Quantity

Sold only to research institutions and qualified laboratory professionals. Not for sale to individuals for personal use.

Pfu DNA Polymerase is a thermo-stable enzyme having a Mw of about 90kDa. Pfu DNA Polymerase is derived from E. coli that and cloned from Pyrococcus furiosus strain Vc1 DSM3638.

  • Expression host: Escherichia Coli.
  • Applications: 1. Ideal for high-fidelity amplification. 2. 3'-5' exonuclease activity provides a low error rate. 3. One of the most thermostable DNA polymerases known. 4. Lack of extendase activity means no unwanted 3 overhangs. 5. Optimal for blunt-end PCR cloning. 6.
  • Formulation: 50mM Tris-HCl, pH 8.2, 1mM DTT, 0.1mM EDTA, 0.05% CHAPS and 50% glycerol.
  • Appearance: Sterile liquid formulation.
  • Stability: Pfu DNA Polymerase although stable at 10°C for 5 days, should be stored below -18°C.Please prevent freeze-thaw cycles.

Catalog number PT_41744. Available sizes: 100U, 500U, 2500U. Order online or request a quote. For research use only. Not for diagnostic or therapeutic use, and not for human or animal consumption.

Data sheet

Formulation
50mM Tris-HCl, pH 8.2, 1mM DTT, 0.1mM EDTA, 0.05% CHAPS and 50% glycerol.
Applications
1. Ideal for high-fidelity amplification. 2. 3'-5' exonuclease activity provides a low error rate. 3. One of the most thermostable DNA polymerases known. 4. Lack of extendase activity means no unwanted 3 overhangs. 5. Optimal for blunt-end PCR cloning. 6.
Description
Pfu DNA Polymerase is a thermo-stable enzyme having a Mw of about 90kDa. Pfu DNA Polymerase is derived from E. coli that and cloned from Pyrococcus furiosus strain Vc1 DSM3638. Pfu DNA Polymerase replicates DNA at 75°C, catalyzing the polymerization of nu
Protein Background
Pfu DNA polymeraseenzyme is found in the hyperthermophilic archaeonPyrococcus furiosus, where it functions in vivoto replicate the organism's DNA. In vitro, Pfu is used to swiftly amplify DNAin the Polymerase Chain Reaction, where the enzyme serves the ce
Expression host
Escherichia Coli.
Synonyms
DNA polymerase, EC 2.7.7.7, Pfu polymerase, Pfu-DNA Polymerase.
Reagent Appearance
Sterile liquid formulation.
Stability
Pfu DNA Polymerase although stable at 10°C for 5 days, should be stored below -18°C.Please prevent freeze-thaw cycles.
Unit Definition
1U of enzyme catalyzes the incorporation of 10nmol of dNTP into acid-insoluble product in 30 minutes at 75°C.
Product Description
1. Binds with greater affinity to most mammalian immunoglobulins than Protein A, including human IgG3 and rat IgG2a.2. Does not bind to human IgM, IgD and IgA.
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