Data sheet
| Assay Range | 31.25 - 2000 pg/ml |
More info
Sensitivity
30 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant Rat ICAM-1 standard: 5 ng/vial - 2.
2. One 96-well plate precoated with anti-Rat ICAM-1 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Rat ICAM-1 Ab: 1 vial, dilution 1:1000.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
ICAM1 (Inter-Cellular Adhesion Molecule 1), also known as CD54, is a type of intercellular adhesion molecule. It is a cell surface glycoprotein which is typically expressed onendothelial cells and cells of the immune system. It binds to integrins of typeCD11a / CD18, or CD11b / CD18 and is also exploited by rhinovirus as a receptor.Its expression is activated by p53 in an NF-kappaB-independent manner and induced by TNFalpha in a process that involves IKK beta.
