Rat ICAM-1 / CD54 ELISA Kit (NS-E10184)
search
  • Rat ICAM-1 / CD54 ELISA Kit (NS-E10184)

Rat ICAM-1 / CD54 ELISA Kit

Catalog # NS-E10184

Print

$625.00
Quantity

Sensitivity
30 pg/mL
 
Size
96T
 
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
 
Assay Principle
Sandwich ELISA
 
Sample volume
100 µL final volume, dilution factor varies on samples.
 
Detection Method
Chromogenic
 
 
 
 
Kit Components
 
 
 
1. Recombinant Rat ICAM-1 standard: 5 ng/vial - 2.
 
 
2. One 96-well plate precoated with anti-Rat ICAM-1 Ab
 
 
3. Sample diluent buffer: 12 mL x 1
 
 
4. HRP conjugated anti-Rat ICAM-1 Ab: 1 vial, dilution 1:1000.
 
 
5. Detection Antibody diluent buffer: 12 mL x 1
 
 
6. 20 x Wash Solution: 25 ml x 1.
 
 
7. TMB color developing agent A: 6 ml.
 
 
8. TMB color developing agent B: 6 ml.
 
 
9. TMB stop solution: 6ml.

 

Background

 

ICAM1 (Inter-Cellular Adhesion Molecule 1), also known as CD54, is a type of intercellular adhesion molecule. It is a cell surface glycoprotein which is typically expressed onendothelial cells and cells of the immune system. It binds to integrins of typeCD11a / CD18, or CD11b / CD18 and is also exploited by rhinovirus as a receptor.Its expression is activated by p53 in an NF-kappaB-independent manner and induced by TNFalpha in a process that involves IKK beta.

Standard Curve

Data sheet

Assay Range
31.25 - 2000 pg/ml
New