Mouse IL6RA / CD126 ELISA Kit

Catalog # NS-E10175

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$625.00
Quantity

Sensitivity
5 pg/mL
 
Size
96T
 
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
 
Assay Principle
Sandwich ELISA
 
Sample volume
100 µL final volume, dilution factor varies on samples.
 
Detection Method
Chromogenic
 
 
 
 
Kit Components
 
 
 
1. Recombinant Mouse IL6RA standard: 1 ng/vial - 2.
 
 
2. One 96-well plate precoated with anti-Mouse IL6RA Ab
 
 
3. Sample diluent buffer: 12 mL x 1
 
 
4. HRP conjugated anti-Mouse IL6RA Ab: 1 vial, dilution 1:500.
 
 
5. Detection Antibody diluent buffer: 12 mL x 1
 
 
6. 20 x Wash Solution: 25 ml x 1.
 
 
7. TMB color developing agent A: 6 ml.
 
 
8. TMB color developing agent B: 6 ml.
 
 
9. TMB stop solution: 6ml.

 

Background

 

Interleukin 6 receptor (IL-6R) also known as CD126 (Cluster of Differentiation 126) is a type I cytokine receptor. Interleukin 6 (IL6) is a potent pleiotropic cytokine that regulates cell growth and differentiation and plays an important role in immune response. Dysregulated production of IL6 and this receptor are implicated in the pathogenesis of many diseases, such as multiple myeloma, autoimmune diseases and prostate cancer. IL-6R binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitates an association with IL6ST. Activation may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis. Interleukin-6 receptor has been shown to interact with Interleukin 6 and Ciliary neurotrophic factor. High levels of IL-6 and sIL-6R have been reported in several chronic inflammatory and autoimmune diseases as well as in cancer.

Standard Curve

Data sheet

Assay Range
6.25 - 400 pg/ml
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