Mouse B7-1/CD80 ELISA Kit

Kit Components

 1. Recombinant Mouse B7-1 standard: 2 vials

 2. One 96-well plate precoated with anti-Mouse B7-1 Ab

 3. Sample diluent buffer: 12 mL - 1

 4. Detection antibody: 130 µL, dilution 1:100

 5. Streptavidin-HRP: 130 µL, dilution 1:100

 6. Antibody diluent buffer: 12 mL x1   

 7. Streptavidin-HRP diluent buffer: 12 mL x1

 8. TMB developing agent: 10 mL x1

9. Stop solution: 10 mL x1.

10. Washing solution (20x): 25 mL x1.

Background

B7-1, also known as T-lymphocyte activation antigen CD80, Activation B7-1 antigen, BB1, CTLA-4 counter-receptor B7.1, or CD80, is a type I transmembrane protein belonging to the Immunoglobulin superfamily. Mouse B7-1 is a 306 amino acid (aa) protein containing a putative 37 aa signal peptide, a 190 aa extracellular domain, a 22 aa transmembrane domain, and a 38 aa cytoplasmic domain. Mouse B7-1 shares 28% aa identity with mouse B7-2. Mouse B7-1 is 44% aa identical to its human counterpart. B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on inter-digitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells.

B7-1 and B7-2 are the ligands for CD28 and CTLA4. They constitute one of the dominant costimulatory pathways that regulate T and B cell responses and are essential for T cell activation and survival. CTLA4 binds to B7-1 and B7-2 with a much higher affinity than CD28 and is involved in the down-regulation of the immune response.