Human vWF ELISA Kit ( von Willebrandfactor )
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  • Human vWF ELISA Kit ( von Willebrandfactor )

Human vWF ELISA Kit ( von Willebrandfactor )

Catalog # NS-E10153

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$625.00
Quantity

Sensitivity
150 pg/mL
 
Size
96T
 
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
 
Assay Principle
Sandwich ELISA
 
Sample volume
100 µL final volume, dilution factor varies on samples.
 
Detection Method
Chromogenic
 
 
 
 
Kit Components
 
 
 
1. Recombinant human vWF standard: 10 ng/vial - 2.
 
 
2. One 96-well plate precoated with anti-human vWF Ab
 
 
3. Sample diluent buffer: 12 mL x 1
 
 
4. Biotin conjugated anti-Human vWF Ab: 1 vial, dilution 1:500.
 
 
5. Detection Antibody diluent buffer: 12 mL x 2
 
 
6. Streptavidin-HRP: 60µL, dilution 1:200.
 
 
7. TMB color developing agent A: 6 ml.
 
 
8. TMB color developing agent B: 6 ml.
 
 
9. TMB stop solution: 6ml.

 

Background

 

Von Willebrand factor (vWF) is a blood glycoprotein involved in hemostasis. It is deficient or defective in von Willebrand disease and is involved in a large number of other diseases, including thrombotic thrombocytopenic purpura, Heyde's syndrome, and possibly hemolytic-uremic syndrome. Increased plasma levels in a large number of cardiovascular, neoplastic, and connective tissue diseases are presumed to arise from adverse changes to the endothelium, and may contribute to an increased risk of thrombosis.

Standard Curve

 

 

Data sheet

Assay Range
156 - 10000 pg/ml
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