Data sheet
| Assay Range | 31.25 - 2000 pg/ml |
More info
Sensitivity
30 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human KNG1 standard: 2 ng/vial - 2.
2. One 96-well plate precoated with anti-human KNG1 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human KNG1 Ab: 1 vial, dilution 1:250.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
Kininogen-1 (KNG1), also known as alpha-2-thiol proteinase inhibitor,Williams-Fitzgerald-Flaujeac factor or the HMWK-kallikrein factor, is a secreted protein which contains three cystatin domains. Kininogen-1 is a constituent of the blood coagulation system as well as the kinin-kallikrein system. Defects in KNG1 are the cause of high molecular weight kininogen deficiency (HMWK deficiency) which is an autosomal recessive coagulation defect. Patients with HWMK deficiency do not have a hemorrhagic tendency, but they exhibit abnormal surface-mediated activation of fibrinolysis.
