Data sheet
| Assay Range | 31.25 - 2000 pg/ml |
More info
Sensitivity
30 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human IL3RA standard: 4 ng/vial - 2.
2. One 96-well plate precoated with anti-human IL3RA Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human IL3RA Ab: 1 vial, dilution 1:500.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
The interleukin-3 receptor (also known as CD123 antigen) is a molecule found on cells which helps transmit the signal of interleukin-3, a soluble cytokine important in the immune system.The receptor belongs to the type I cytokine receptor family and is a heterodimer with a unique alpha chain paired with the common beta (beta c or CDw131) subunit.The receptor induces tyrosinephosphorylation within the cell and promotes proliferation and differentiation within the hematopoietic cell lines.
