Data sheet
| Assay Range | 31.25 - 2000 pg/ml |
More info
Sensitivity
30 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human IL 17RA standard: 4 ng/vial - 2.
2. One 96-well plate precoated with anti-human IL 17RA Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human IL 17 RA Ab: 1 vial, dilution 1:500.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
Interleukin 17 receptor A, also known as IL17RA and CD217 (cluster of differentiation 217), is a proinflammatory cytokine secreted by activated T-lymphocytes. IL17RA is a potent inducer of the maturation of CD34+ hematopoietic precursors into neutrophils. IL17RA is a ubiquitous type I membrane glycoprotein that binds with low affinity to interleukin 17A. Interleukin 17A and its receptor play a pathogenic role in many inflammatory and autoimmune diseases such as rheumatoid arthritis. Like other cytokine receptors, this receptor likely has a multimeric structure.
