Data sheet
| Assay Range | 46.88 - 3000 pg/ml |
More info
Sensitivity
40 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human EpCAM standard: 5 ng/vial - 2.
2. One 96-well plate precoated with anti-human EpCAM Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human EpCAM Ab: 1 vial, dilution 1:500.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
EPCAM is a single-pass type I membrane protein of the EPCAM family. EPCAM is expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. EPCAM is intricately linked with the Cadherin-Catenin pathway and hence the fundamental WNT pathwayresponsible for intracellular signalling and polarity. It has been used as an immunotherapeutic target in the treatment of gastrointestinal, urological and other carcinomas.
